The Interaction between Raspberry Ringspot and Tobacco Rattle Viruses in Doubly Infected Protoplasts

@article{Barker1977TheIB,
  title={The Interaction between Raspberry Ringspot and Tobacco Rattle Viruses in Doubly Infected Protoplasts},
  author={Hugh Barker and Bryan D. Harrison},
  journal={Journal of General Virology},
  year={1977},
  volume={35},
  pages={135-148}
}
Summary Tobacco mesophyll protoplasts infected with raspberry ringspot virus (RRV) show faint generalized staining when treated with fluorescent antibody to RRV, but many brightly staining granules develop after inoculation with a mixture of RRV and the CAM strain of tobacco rattle virus (TRV-CAM). The phenomenon occurred with three different strains of RRV but not when tobacco mosaic virus, or two other strains of tobacco rattle virus, were substituted for the CAM strain. The RRV antigen… 
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Infection of Tobacco Mesophyll Protoplasts with Raspberry Ringspot Virus alone and together with Tobacco Rattle Virus
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Infection of Tobacco Mesophyll Protoplasts with Raspberry Ringspot Virus alone and together with Tobacco Rattle Virus
TLDR
Raspberry ringspot virus (RRV) infected tobacco mesophyll protoplasts best, judged by the yield of infective virus, when the inoculation mixture contained 0.04 to 5 µg/ml virus, and many of the protoplast inoculated with mixtures of RRV and the CAM strain of tobacco rattle virus (TRV-CAM) contained numerous discrete granules ofRRV particle antigen throughout the cytoplasm.
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