The Influence of MS Imaging Parameters on UV-MALDI Desorption and Ion Yield

@article{Robinson2019TheIO,
  title={The Influence of MS Imaging Parameters on UV-MALDI Desorption and Ion Yield},
  author={Kenneth N Robinson and Rory T. Steven and Alan M. Race and Josephine Bunch},
  journal={Journal of The American Society for Mass Spectrometry},
  year={2019},
  pages={1-10}
}
AbstractUltraviolet matrix-assisted laser desorption/ionization mass spectrometry imaging (UV-MALDI MSI) is a widely used technique for imaging molecular distributions within biological systems. While much work exists concerning desorption in UV-MALDI MS, the effects of commonly varied parameters for imaging applications (repetition rate, use of continuous raster mode and raster speed), which determine spatial resolution and limits of detection for the technique, remain largely unknown. We use… Expand
2 Citations
Detailed Characterization of the Post-Ionization Efficiencies in MALDI-2 as a Function of Relevant Input Parameters.
TLDR
A semi-automatic LabVIEW-based protocol is used to investigate and optimize the efficiency of the PI process in dependence of four relevant input parameters and a dense parameter grid: pulse energies of the two lasers, delay between the laser pulses, and buffer gas pressure in the ion source. Expand
LASER DESORPTION/ABLATION POSTIONIZATION MASS SPECTROMETRY: RECENT PROGRESS IN BIOANALYTICAL APPLICATIONS.
TLDR
This review is intended to provide a timely comprehensive view of the broadening bioanalytical applications of disparate LD/API techniques, according to the classifications based on the postionization methods. Expand

References

SHOWING 1-10 OF 67 REFERENCES
Investigating MALDI MSI parameters (Part 1) - A systematic survey of the effects of repetition rates up to 20kHz in continuous raster mode.
TLDR
The interrelated influence of varying laser energy, repetition rate and stage raster speed on detected ion intensity is reported for the first time and a clear dependence on time between laser pulses is observed indicating the underlying mechanisms may be related to on-plate hysteresis-exhibiting processes such as matrix chemical modification. Expand
Scanning microprobe matrix-assisted laser desorption ionization (SMALDI) mass spectrometry: Instrumentation for sub-micrometer resolved LDI and MALDI surface analysis
TLDR
A new instrument and method is described for laterally resolved mass spectrometric surface analysis, including first applicational examples in elemental analysis, imaging of pine tree roots, and investigation of MALDI sample morphology in biomolecular analysis. Expand
LIF-imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes
The first gated LIF-imaging and absorption spectroscopy has been performed on laser desorbed plumes from organic crystals which are commonly used as MALDI (Matrix Assisted Laser DesorptionExpand
LIF-imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes
The first gated LIF-imaging and absorption spectroscopy has been performed on laser desorbed plumes from organic crystals which are commonly used as MALDI (Matrix Assisted Laser DesorptionExpand
Fluorometric Beam Profiling of UV MALDI Lasers
TLDR
In this study, area estimation by ablation provided a value 1.6 times smaller than that obtained by the fluorometric method, demonstrating the need for caution when measuring beam profile and, therefore, fluence, in MALDI-MS. Expand
Probing the Relationship Between Detected Ion Intensity, Laser Fluence, and Beam Profile in Thin Film and Tissue in MALDI MSI
TLDR
The detected ion intensity versus fluence relationship was investigated, for the first time, in both thin films of α-cyano-4-hydroxycinnamic acid with phosphatidylcholine 34:1 lipid standard and in CHCA coated murine tissue sections for both the square and round optic fibers in continuous raster imaging mode. Expand
New insights into mechanisms of material ejection in MALDI mass spectrometry for a wide range of spot sizes
TLDR
Results on the influence of laser fluence and spot size on the mechanisms of the initial material ejection in MALDI and subsequent plume development reveal a peculiar influence of the Spot size on analyte fragmentation as well as plumeDevelopment and allows for new insights into the unexplained spot size effect reported for MAL DI. Expand
Color Matters—Material Ejection and Ion Yields in UV-MALDI Mass Spectrometry as a Function of Laser Wavelength and Laser Fluence
TLDR
The material ejection as probed by the photoacoustic method is excellently fitted by the quasithermal model, while a sigmoidal function allows for an empirical description of the ion signal–fluence relationship. Expand
Matrix Optical Absorption in UV-MALDI MS
TLDR
The first absolute solid phase spectra of the matrix compounds α-cyano-4-hydroxycinnamic acid, para-nitroaniline, PNA, 2-mercaptobenzothiazole, MBT, and THAP are reported; additionally, inconsistencies between previously published spectra for CHCA are resolved. Expand
Investigation of the Desorption Process in UV Matrix-Assisted Laser Desorption/Ionization with a Liquid 3-Nitrobenzyl Alcohol Matrix by Photoacoustic Analysis, Fast-Flash Imaging, and UV-Laser Postionization†
Ultraviolet matrix-assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) is a widely used method for the analysis of a variety of biomolecules. The technique relies on the codesorptionExpand
...
1
2
3
4
5
...