Corpus ID: 85753738

The Influence of Glycation in Three-Dimensional Human Cultured Epidermis

  title={The Influence of Glycation in Three-Dimensional Human Cultured Epidermis},
  author={R. Ichijo and Y. Niki and M. Yagi and M. Ichihashi and K. Nomoto and Y. Yonei},
Destination: The influence by the addition of glucose and glyoxal was studied in three-dimensional human cultured epidermis. Methods: The glucose concentration (3-5g/L) of the medium for a three-dimensional human cultured epidermis (MEL-300) was changed and AGEs intermediate, glyoxal (GO)(0.3 μg/mL) were added, and it was cultivated at 37°C for 14 days. The amount of protein, N e -(carboxymethyl) lysine (CML), pentosidine and fluorescence intensity from AGEs of the tissue homogenate was… Expand
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