The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing

@inproceedings{Kennedy2014TheIO,
  title={The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing},
  author={N A Kennedy and Alan W Walker and Susan H. Berry and S. H. Duncan and Freda M. Farquarson and Petra Louis and John Michael Thomson and Jack Satsangi and Harry James Flint and Julian Parkhill and Charlie W. Lees and Georgina L Hold and Taimur Ahmad and CA Anderson and J Barrett and Hazel E. Drummond and Caitlin Edwards and Alisa Hart and Christopher G Hawkey and Paul Henderson and Mohid Shakil Khan and CA Lamb and J C Lee and JC Mansfield and C H Mathew and Craig Mowat and W. Newman and NJ Prescott and Alison Simmons and Paul W. Simpson and Kristen H. Taylor and D Wilson},
  booktitle={PloS one},
  year={2014}
}
INTRODUCTION Determining bacterial community structure in fecal samples through DNA sequencing is an important facet of intestinal health research. The impact of different commercially available DNA extraction kits upon bacterial community structures has received relatively little attention. The aim of this study was to analyze bacterial communities in volunteer and inflammatory bowel disease (IBD) patient fecal samples extracted using widely used DNA extraction kits in established… CONTINUE READING
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A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samples

  • S Claassen, E du Toit, M Kaba, C Moodley, HJ Zar
  • J Microbiol Methods
  • 2013
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