The Immobiline family: from "vacuum" to "plenum" chemistry.

  title={The Immobiline family: from "vacuum" to "plenum" chemistry.},
  author={Marcella Chiari and Pier Giorgio Righetti},
  volume={13 4},
We list here a total of 17 acrylamido acids and bases as potential buffers and titrants for isoelectric focusing separations in immobilized pH gradients. The chemistry of these compounds is reviewed and general guidelines are given for their proper use. In particular, it is shown that the most delicate compounds are the basic species, since they can undergo several degradation pathways, including: (i) spontaneous hydrolysis to acrylic acid and a diamine; (ii) spontaneous autopolymerization to… CONTINUE READING