The IgE repertoire in PBMCs of atopic patients is characterized by individual rearrangements without variable region of the heavy immunoglobulin chain bias.

@article{Lim2007TheIR,
  title={The IgE repertoire in PBMCs of atopic patients is characterized by individual rearrangements without variable region of the heavy immunoglobulin chain bias.},
  author={Annick Lim and Stephan Luderschmidt and Anke Weidinger and Christina Schnopp and Johannes Hofaker Ring and R{\"u}diger Hein and Markus W. Ollert and Martin Mempel},
  journal={The Journal of allergy and clinical immunology},
  year={2007},
  volume={120 3},
  pages={696-706}
}
BACKGROUND Patients with atopic diseases are characterized by high levels of specific IgE production. However, little is known about the composition of their B-cell repertoires. OBJECTIVES We sought to analyze the complete PBMC-derived IgE repertoire and to compare clonal expansions between different patients. METHODS We have analyzed the IgE-bearing B-cell receptor repertoire in highly atopic patients (>1000 IU/mL) using quantitative RT-PCR, complementarity determining region 3… CONTINUE READING