The IRG mouse: a two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ.

@article{Gasperi2008TheIM,
  title={The IRG mouse: a two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ.},
  author={Rita De Gasperi and Anne B. Rocher and Miguel A. Gama Sosa and Susan L. Wearne and Gissel M Perez and Victor L. Jr. Friedrich and Patrick R. Hof and Gregory A Elder},
  journal={Genesis},
  year={2008},
  volume={46 6},
  pages={308-17}
}
The Cre-loxP system is widely used for making conditional alterations to the mouse genome. Cre-mediated recombination is frequently monitored using reporter lines in which Cre expression activates a reporter gene driven by a ubiquitous promoter. Given the distinct advantages of fluorescent reporters, we developed a transgenic reporter line, termed IRG, in which DsRed-Express, a red fluorescent protein (RFP) is expressed ubiquitously prior to Cre-mediated recombination and an enhanced green… CONTINUE READING

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