The I-CreI meganuclease and its engineered derivatives: applications from cell modification to gene therapy.

@article{Arnould2011TheIM,
  title={The I-CreI meganuclease and its engineered derivatives: applications from cell modification to gene therapy.},
  author={S Arnould and Christophe Delenda and Sylvestre Grizot and Carole Desseaux and Fr{\'e}d{\'e}ric P{\^a}ques and George H. Silva and Julianne Smith},
  journal={Protein engineering, design & selection : PEDS},
  year={2011},
  volume={24 1-2},
  pages={27-31}
}
Meganucleases (MNs) are highly specific enzymes that can induce homologous recombination in different types of cells, including mammalian cells. Consequently, these enzymes are used as scaffolds for the development of custom gene-targeting tools for gene therapy or cell-line development. Over the past 15 years, the high resolution X-ray structures of several MNs from the LAGLIDADG family have improved our understanding of their protein-DNA interaction and mechanism of DNA cleavage. By… CONTINUE READING