The Human Red Blood Cell Proteome and Interactome
@article{Goodman2007TheHR, title={The Human Red Blood Cell Proteome and Interactome}, author={Steven R. Goodman and Anastasia Kurdia and Larry P. Ammann and David G. Kakhniashvili and Ovidiu Daescu}, journal={Experimental Biology and Medicine}, year={2007}, volume={232}, pages={1391 - 1408} }
The red blood cell or erythrocyte is easily purified, readily available, and has a relatively simple structure. Therefore, it has become a very well studied cell in terms of protein composition and function. RBC proteomic studies performed over the last five years, by several laboratories, have identified 751 proteins within the human erythrocyte. As RBCs contain few internal structures, the proteome will contain far fewer proteins than nucleated cells. In this minireview, we summarize the…
164 Citations
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References
SHOWING 1-10 OF 41 REFERENCES
In-depth analysis of the membrane and cytosolic proteome of red blood cells.
- BiologyBlood
- 2006
The data reveal unexpected complexity of the RBC proteome, provide a wealth of data on its composition, shed light on several open issues in RBC biology, and form a departure point for comprehensive understanding of RBC functions.
The Human Erythrocyte Proteome
- BiologyMolecular & Cellular Proteomics
- 2004
An analysis of the red blood cell proteome by ion trap tandem mass spectrometry in line with liquid chromatography found 181 unique protein sequences, including membrane skeletal proteins, metabolic enzymes, transporters and channel proteins, adhesion proteins, hemoglobins, cellular defense proteins, proteins of the ubiquitin-proteasome system, and others.
The Proteomics of Sickle Cell Disease: Profiling of Erythrocyte Membrane Proteins by 2D-DIGE and Tandem Mass Spectrometry
- BiologyExperimental biology and medicine
- 2005
Elevated content of protein repair participants as well as oxygen radical scavengers may reflect the increased oxidative stress observed in sickle cells.
Proteomics approach reveals novel proteins on the surface of malaria-infected erythrocytes.
- BiologyMolecular and biochemical parasitology
- 2004
Red blood cell membrane defects.
- MedicineReviews in clinical and experimental hematology
- 2003
An overview of the currently known molecular basis of red cell membrane disorders is presented and dehydrated hereditary stomatocytosis was shown to belong to a pleiotropic syndrome: DHS + fetal edema + pseudohyperkalemia, which maps 16q23-24.
Spectrin-based skeleton in red blood cells and malaria
- BiologyCurrent opinion in hematology
- 2007
Certain instances of spectrin mutations or polymorphisms have not yet been shown to constitute new factors of innate resistance to malaria in vivo, so the relationships between parasite and spectrin-based skeleton should be examined more closely and the molecular interactions between parasite ligands and particular spectrin chain domains should be characterized.
Update on the clinical spectrum and genetics of red blood cell membrane disorders.
- Medicine, BiologyCurrent hematology reports
- 2004
Six genetic loci have been identified and many defects, including gene deletions and insertions, missense and nonsense mutations, and splicing mutations, have been found and provided a better understanding of the pathogenesis of these disorders.
2-D DIGE analysis of liver and red blood cells provides further evidence for oxidative stress in schizophrenia.
- Psychology, MedicineJournal of proteome research
- 2007
Investigation of liver tissue and red blood cells from schizophrenia patients and controls using 2-D DIGE proteomic analysis corroborate the earlier findings obtained from post-mortem brain studies and substantiate the hypothesis that metabolic alterations leading to oxidative stress are linked to the schizophrenia disease process.
The spectrin membrane skeleton of normal and abnormal human erythrocytes: a review.
- BiologyThe American journal of physiology
- 1983
This review shall give an historical development of the current model of the erythrocyte membrane skeleton, and describe how the experimental technology developed to study the normal membrane skeleton has paved the way for the recent identification of alterations of skeletal protein interactions in hereditary spherocytosis, hereditary elliptocyTosis, and hereditary pyropoikilocytosis.
Protein profiling of sickle cell versus control RBC core membrane skeletons by ICAT technology and tandem mass spectrometry
- BiologyCellular & Molecular Biology Letters
- 2006
A proteomic approach using a cleavable ICAT reagent and nano-LC ESI tandem mass spectrometry was used to perform protein profiling of core RBC membrane skeleton proteins between sickle cell patients (SS) and controls (AA), and it was determined that there is no significant difference in the mean ratios.