The Human Interferon-y Receptor PURIFICATION

  title={The Human Interferon-y Receptor PURIFICATION},
  author={Daniela Novick and Patricia L. Orchansky and M. Revel and Menachem Rubinstein},
The receptor for human interferon-y (IFN-y) was purified from foreskin fibroblasts. Triton X-100 extracts obtained from either intact cells or membrane preparations were passed through an immobilized interferon-? column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of eluted fractions revealed a major band of M, = 95,000 and minor bands of M. = 80,000 and 60,000. Further purification was obtained by steric exclusion and by lectin chromatography. The purified receptor… CONTINUE READING


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G. Trinchieri, B. Perussia
Today • 1985