The Hsp82 Molecular Chaperone Can Promote a Switch between Unextendable and Extendable Telomere States

Distinct protein assemblies are nucleated at telomeric DNA to both guard the ends from damage and lengthen the DNA after replication. In yeast, Cdc13 recruits either Stn1-Ten1 to form a protective cap or the telomerase holoenzyme to extend the DNA. We have established an in vitro yeast telomere system in which Stn1-Ten1–unextendable or telomerase-extendable… CONTINUE READING