The Helix-II epitope: a cautionary tale from a cartilage biomarker based on an invalid collagen sequence.

@article{Eyre2009TheHE,
  title={The Helix-II epitope: a cautionary tale from a cartilage biomarker based on an invalid collagen sequence.},
  author={David R. Eyre and Mary Ann Weis},
  journal={Osteoarthritis and cartilage},
  year={2009},
  volume={17 4},
  pages={423-6}
}
OBJECTIVE An apparent database error in the sequence underlying the Helix-II cartilage biomarker immunoassay was investigated at the protein level. METHODS AND RESULTS Tandem mass spectrometry established the peptide sequence ERGETGPP*GPA in human type II collagen, not ERGETGPP*GTS used to generate the antibody for the Helix-II assay. CONCLUSIONS Recent… CONTINUE READING