The Helicase and RNaseIIIa Domains of Arabidopsis Dicer-Like 1 Modulate Catalytic Parameters during MicroRNA Biogenesis 1 [ C ] [ W ] [ OA ]

@inproceedings{Liu2012TheHA,
  title={The Helicase and RNaseIIIa Domains of Arabidopsis Dicer-Like 1 Modulate Catalytic Parameters during MicroRNA Biogenesis 1 [ C ] [ W ] [ OA ]},
  author={Chenggang Liu and Michael J. Axtell and Nina V. Fedoroff},
  year={2012}
}
Dicer-Like1 (DCL1), an RNaseIII endonuclease, and Hyponastic Leaves1 (HYL1), a double-stranded RNA-binding protein, are core components of the plant microRNA (miRNA) biogenesis machinery. hyl1 null mutants accumulate low levels of miRNAs and display pleiotropic developmental phenotypes. We report the identification of five new hyl1 suppressor mutants, all of which are alleles of DCL1. These new alleles affect either the helicase or the RNaseIIIa domains of DCL1, highlighting the critical… CONTINUE READING
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