The H,K-ATPase beta subunit as a model to study the role of N-glycosylation in membrane trafficking and apical sorting.

@article{Vagin2004TheHB,
  title={The H,K-ATPase beta subunit as a model to study the role of N-glycosylation in membrane trafficking and apical sorting.},
  author={Olga Vagin and Shahlo Turdikulova and George Sachs},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 37},
  pages={39026-34}
}
The role of N-glycosylation in trafficking of an apical membrane protein, the gastric H,K-ATPase beta subunit linked to yellow fluorescent protein, was analyzed in polarized LLC-PK1 cells by confocal microscopy and surface-specific biotinylation. Deletion of the N-glycosylation sites at N1, N3, N5, and N7 but not at N2, N4, and N6 significantly slowed endoplasmic reticulum-to-Golgi trafficking, impaired apical sorting, and enhanced endocytosis from the apical membrane, resulting in decreased… CONTINUE READING

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