The Gs-Linked Receptor GPR3 Maintains Meiotic Arrest in Mammalian Oocytes

@article{Mehlmann2004TheGR,
  title={The Gs-Linked Receptor GPR3 Maintains Meiotic Arrest in Mammalian Oocytes},
  author={Lisa M. Mehlmann and Yoshinaga Saeki and Shigeru Tanaka and T. Brennan and Alexei V. Evsikov and Frank L. Pendola and Barbara B. Knowles and John J. Eppig and Laurinda A Jaffe},
  journal={Science},
  year={2004},
  volume={306},
  pages={1947 - 1950}
}
Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype can be reversed by injection of Gpr3 RNA into the oocytes. Thus, the GPR3 receptor is a link in communication between the somatic cells and… 
Oocyte-specific expression of Gpr3 is required for the maintenance of meiotic arrest in mouse oocytes.
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Results show that endocytosis occurs in the oocyte, leading to a decrease in cAMP production, and suggest that there is a balance between cAMPProduction and degradation in the arrested oocyte that maintains cAMP levels at an appropriate level during the maintenance of meiotic arrest.
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  • P. Thomas
  • Biology
    The Journal of Steroid Biochemistry and Molecular Biology
  • 2017
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