The Graafian Follicle Is a Site of L-Ascorbate Accumulation


Purpose: Our purpose was to evaluate the L-ascorbate level in human preovulatory follicular fluid and to quantify the blood/follicle gradient for vitamin C. The effect of smoking on the follicular L-ascorbate concentration was studied. The correlations were tested between follicular L-ascorbate and follicle size and oocyte maturity. Methods: In 65 women undergoing in vitro fertilization, samples of follicular fluid and blood serum were collected. Biochemical analyses included L-ascorbate determinations by a colorimetric method and cotinine measurements by a radioimmunoassay. Results: The average follicular fluid:serum ratio for L-ascorbate was 1:68. Ascorbate levels in follicular fluid and serum were significantly correlated. The follicular L-ascorbate level did not correlate with the follicle size and the oocyte maturity grade. Insignificantly lowered follicular L-ascorbate levels were observed in smokers. Conclusions: The extracellular compartment of the Graafian follicle is a site of an ascorbate accumulation. Exposure to tobacco smoke does not significantly diminish the intrafollicular pool of L-ascorbate.

DOI: 10.1023/A:1022597629622

3 Figures and Tables


Citations per Year

96 Citations

Semantic Scholar estimates that this publication has 96 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Paszkowski1999TheGF, title={The Graafian Follicle Is a Site of L-Ascorbate Accumulation}, author={Tomasz Paszkowski and Robert N. Clarke}, journal={Journal of Assisted Reproduction and Genetics}, year={1999}, volume={16}, pages={41-45} }