The Genome Sequence of the Malaria Mosquito Anopheles gambiae

@article{Holt2002TheGS,
  title={The Genome Sequence of the Malaria Mosquito Anopheles gambiae},
  author={Robert A. Holt and G. M. Subramanian and Aaron L. Halpern and Granger G. Sutton and Rosane Charlab and Deborah R. Nusskern and Patrick Wincker and Andrew G. Clark and JoséM. C. Ribeiro and Ron Wides and Steven L. Salzberg and Brendan J. Loftus and Mark Yandell and William H. Majoros and Douglas B. Rusch and Zhongwu Lai and Cheryl L. Kraft and Josep F. Abril and V{\'e}ronique Anthouard and Peter Arensburger and Peter W Atkinson and H. Maria Baden and V{\'e}ronique de Berardinis and Danita Baldwin and Vladim{\'i}r Bene{\vs} and James Kite Biedler and Claudia Blass and Randall A. Bolanos and Didier Boscus and Mary Barnstead and Shuang Cai and Angela Center and Kabir Chatuverdi and George K. Christophides and Mathew A Chrystal and Michele E. Clamp and Anibal Cravchik and Val Curwen and Ali N Dana and Arthur L. Delcher and Ian M. Dew and Cheryl A. Evans and Michael Flanigan and Anne Grundschober-Freimoser and Lisa Friedli and Zhiping Gu and Ping Guan and Roderic Guig{\'o} and Maureen Hillenmeyer and Susanne L Hladun and James R. Hogan and Young S. Hong and Jeffrey Hoover and Olivier Jaillon and Zhaoxi Ke and Chinnappa D. Kodira and Elena Kokoza and Anastasios C. Koutsos and Ivica Letunic and Alexander Levitsky and Yong Liang and Jhy-Jhu Lin and Neil F. Lobo and John Lopez and Joel A. Malek and Tina C. McIntosh and Stephan Meister and Jason Rafe Miller and Clark M. Mobarry and Emmanuel Mongin and Sean Murphy and David A. O’brochta and Cynthia Pfannkoch and R. Qi and Megan A. Regier and Karin A. Remington and Hongguang Shao and Maria V. Sharakhova and Cynthia D. Sitter and Jyoti Shetty and Thomas J. Smith and Renee Strong and Jingtao Sun and Dana Thomasova and Lucas Q. Ton and Pantelis Topalis and Zhijian Jake Tu and Maria F. Unger and Brian P. Walenz and Aihui Wang and Jian Wang and Mei Wang and Xuelan Wang and Kerry J. Woodford and Jennifer R. Wortman and Martin Wu and Alison Yao and Evgeny M. Zdobnov and Hongyu Zhang and Qi Zhao and Shaying Zhao and Shiaoping C. Zhu and Igor F. Zhimulev and Mario Coluzzi and Alessandra della Torre and Charles W. Roth and Christos Louis and Francis Kalush and Richard J. Mural and Eugene Wimberly Myers and Mark D. Adams and Hamilton O. Smith and Samuel E. Broder and Malcolm J. Gardner and Claire M. Fraser and Ewan Birney and Peer Bork and Paul T. Brey and J. Craig Venter and Jean Weissenbach and Fotis C. Kafatos and Frank H. Collins and Stephen L. Hoffman},
  journal={Science},
  year={2002},
  volume={298},
  pages={129 - 149}
}
Anopheles gambiae is the principal vector of malaria, a disease that afflicts more than 500 million people and causes more than 1 million deaths each year. Tenfold shotgun sequence coverage was obtained from the PEST strain of A. gambiae and assembled into scaffolds that span 278 million base pairs. A total of 91% of the genome was organized in 303 scaffolds; the largest scaffold was 23.1 million base pairs. There was substantial genetic variation within this strain, and the apparent existence… 
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Title The Genome of Anopheles darlingi , the main neotropical malaria vector Permalink
TLDR
This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which to contemplate the evolutionary history of anopheline mosquitoes, and provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent.
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This study has used new approaches to improve the physical map and assembly of the A. gambiae genome by placing 15 unmapped scaffolds in the pericentromeric regions of the chromosomes and oriented a further 9 scaffolds.
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