The Genoa 2005 outbreak. Determination of putative palytoxin in Mediterranean Ostreopsis ovata by a new liquid chromatography tandem mass spectrometry method.

@article{Ciminiello2006TheG2,
  title={The Genoa 2005 outbreak. Determination of putative palytoxin in Mediterranean Ostreopsis ovata by a new liquid chromatography tandem mass spectrometry method.},
  author={P. Ciminiello and C. Dell’Aversano and E. Fattorusso and M. Forino and G. S. Magno and L. Tartaglione and Claudio Grillo and N. Melchiorre},
  journal={Analytical chemistry},
  year={2006},
  volume={78 17},
  pages={
          6153-9
        }
}
A new method for sensitive, specific, and direct determination of palytoxin is proposed herein. It is based on combination of reversed-phase liquid chromatography with mass spectrometry (LC-MS). The new method was set up on a turbo ion spray-triple quadrupole MS instrument operating in selected ion monitoring (SIM) and multiple reaction monitoring (MRM) acquisition modes (positive ions). The minimum detection levels for matrix-free toxin on column were thus estimated from the data to be 200 and… Expand
New palytoxin-like molecules in Mediterranean Ostreopsis cf. ovata (dinoflagellates) and in Palythoa tuberculosa detected by liquid chromatography-electrospray ionization time-of-flight mass spectrometry.
A rapid, high resolution liquid chromatography coupled with ElectroSpray Ionization Time-Of-Flight Mass Spectrometry (ESI/TOF/MS) method was developed for the determination of the toxin pattern inExpand
Palytoxin in seafood by liquid chromatography tandem mass spectrometry: investigation of extraction efficiency and matrix effect
TLDR
The best conditions for both extraction from seafood and palytoxin quantification by using liquid chromatography tandem mass spectrometry (LC-MS/MS) were investigated and extraction with MeOH/H2O 8:2 provided the best results in terms of accuracy and matrix interference on LC- MS/MS detection of palyToxin. Expand
Complex palytoxin-like profile of Ostreopsis ovata. Identification of four new ovatoxins by high-resolution liquid chromatography/mass spectrometry.
TLDR
A quantitative study of the O. ovata culture extract indicated that the whole of the new ovatoxins represents about 46% of the total toxin content and, thus, their presence has to be taken into account when LC/MS-based monitoring programs of either plankton or contaminated seafood are carried out. Expand
Liquid chromatography–high-resolution mass spectrometry for palytoxins in mussels
TLDR
A procedure including a one-step extraction, solid-phase-extraction (SPE) clean-up, and liquid chromatography-high resolution mass spectrometry (LC–HRMS) detection of individual palytoxins in mussels proved to be efficient chromatographic separation of individual compounds, including structural isomers, with good sensitivity, reproducibility, and linearity in a large dynamic range. Expand
Putative palytoxin and its new analogue, ovatoxin-a, in Ostreopsis ovata collected along the ligurian coasts during the 2006 toxic outbreak
TLDR
This article reports on the liquid chromatography tandem mass spectrometry investigation of plankton samples collected in the summer of 2006 along the Ligurian coasts, coinciding with a massive bloom of the tropical microalga Ostreopsis ovata, and identifies a new palytoxin-like compound never reported so far, which is named ovatoxin-a. Expand
A sensitive assay for palytoxins, ovatoxins and ostreocins using LC-MS/MS analysis of cleavage fragments from micro-scale oxidation.
TLDR
A novel screen approach has been developed that uses LC-MS/MS analysis of substructures generated by oxidative cleavage of vicinal diol groups present in the intact toxin to improve sensitivity for trace analysis and is sufficiently sensitive for regulatory testing, should it be required. Expand
LC-MS/MS analysis of novel ovatoxin isomers in several Ostreopsis strains collected in Japan
TLDR
Detailed toxin profiles of palytoxin analogues in several Japanese Ostreopsis strains were clarified for the first time by liquid chromatography–mass spectrometry (LC–MS). Expand
Simple structural elucidation of ostreocin-B, a new palytoxin congener isolated from the marine dinoflagellate Ostreopsis siamensis, using complementary positive and negative ion liquid chromatography/quadrupole time-of-flight mass spectrometry.
TLDR
The planar structure of ostreocin-B is assigned to 42-hydroxy-3,26-didemethyl-19-deoxypalytoxin (=44-Hydroxyostreoc in-D, and the method used is an excellent tool to obtain structural information on interspecies and intrastrain variation of palyt toxin congeners in marine organisms. Expand
The novel ovatoxin-g and isobaric palytoxin (so far referred to as putative palytoxin) from Ostreopsis cf. ovata (NW Mediterranean Sea): structural insights by LC-high resolution MSn
TLDR
Tentative structures were assigned to both ovatoxin-g and the putative palytoxin, the latter being identified as a palyToxin isomer and re-named as  isobaric paly toxin. Expand
Unique toxin profile of a Mediterranean Ostreopsis cf. ovata strain: HR LC-MS(n) characterization of ovatoxin-f, a new palytoxin congener.
TLDR
The presence of ovatoxin-f should be taken into account when monitoring programs for palytoxin-like compounds in microalgae and/or seawater are carried out. Expand
...
1
2
3
4
5
...