The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan

@inproceedings{Mittal2017TheGT,
  title={The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan},
  author={Nitish Mittal and Joao C Guimaraes and Thomas Gross and Alexander J Schmidt and Arnau Vina-Vilaseca and Danny D. Nedialkova and Florian Aeschimann and Sebastian A Leidel and Anne Spang and Mihaela Zavolan},
  booktitle={Nature Communications},
  year={2017}
}
In Saccharomyces cerevisiae, deletion of large ribosomal subunit protein-encoding genes increases the replicative lifespan in a Gcn4-dependent manner. However, how Gcn4, a key transcriptional activator of amino acid biosynthesis genes, increases lifespan, is unknown. Here we show that Gcn4 acts as a repressor of protein synthesis. By analyzing the messenger RNA and protein abundance, ribosome occupancy and protein synthesis rate in various yeast strains, we demonstrate that Gcn4 is sufficient… CONTINUE READING