The Formation of Extracellular Matrix During Chondrogenic Differentiation of Mesenchymal Stem Cells Correlates with Increased Levels of Xylosyltransferase I

@article{Prante2006TheFO,
  title={The Formation of Extracellular Matrix During Chondrogenic Differentiation of Mesenchymal Stem Cells Correlates with Increased Levels of Xylosyltransferase I},
  author={Christian Prante and Karen Bieback and Carsten Funke and Sylvia Sch{\"o}n and Susanne Kern and Joachim Kuhn and Martin H Gastens and Knut Kleesiek and Christian G{\"o}tting},
  journal={STEM CELLS},
  year={2006},
  volume={24}
}
In vitro differentiation of mesenchymal stem cells (MSCs) into chondrogenic cells and their transplantation is promising as a technique for the treatment of cartilaginous defects. But the regulation of extracellular matrix (ECM) formation remains elusive. Therefore, the objective of this study was to analyze the regulation of proteoglycan (PG) biosynthesis during the chondrogenic differentiation of MSCs. In different stages of chondrogenic differentiation, we analyzed mRNA and protein… Expand
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References

SHOWING 1-10 OF 36 REFERENCES
Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components.
TLDR
The phenotype of the differentiated cell is defined and the sequential events leading from the undifferentiated stem cell to a mature chondrocyte are investigated by analysis of key matrix elements to understand in greater detail the sequential process of matrix assembly. Expand
Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow.
TLDR
Increased understanding of the induction of chondrogenic differentiation should lead to further progress in defining the mechanisms responsible for the generation of cartilaginous tissues, their maintenance, and their regeneration. Expand
Collagen gene expression during chondrogenesis from chick periosteum‐derived cells
TLDR
Collagen gene expression was temporally analyzed at the mRNA level by a reverse transcription PCR procedure, which showed that α1(II) and α1 (IX) collagen mRNAs are coordinately increased, coincident with the onset of overt chondrogenesis, and subsequently decreased as chondrocytes exhibited hypertrophic characteristics. Expand
Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells
  • C. W. Chen, Y. Tsai, +5 authors W. Lai
  • Chemistry, Medicine
  • Journal of orthopaedic research : official publication of the Orthopaedic Research Society
  • 2005
TLDR
Results indicate that type I and II collagen, in addition to the cytokine effect, may play a functional role in regulating of chondrogenic differentiation by MPCs. Expand
The Chondrogenic Potential of Human Bone-Marrow-Derived Mesenchymal Progenitor Cells*
TLDR
The successful induction of in vitro chondrogenesis with human bone-marrow-derived osteochondral progenitor cells is demonstrated in a reliable and reproducible culture system and provides evidence for the clinical utility of these cells in the repair of bone and cartilage. Expand
Expression of the Heparan Sulfate Proteoglycan, Perlecan, during Mouse Embryogenesis and Perlecan Chondrogenic Activity In Vitro
TLDR
It is demonstrated that Pln is not only a marker of chondrogenesis, but also strongly potentiates chondrocytic differentiation in vitro. Expand
Phenotypic and functional comparison of cultures of marrow‐derived mesenchymal stem cells (MSCs) and stromal cells
TLDR
The morphology, phenotype, and in vitro function of cultures of MSCs and traditional marrow‐derived stromal cells (MDSCs) from the same marrow sample are examined to suggest that M SCs represent an important cellular component of the bone marrow microenvironment. Expand
Serum xylosyltransferase: a new biochemical marker of the sclerotic process in systemic sclerosis.
TLDR
The results confirm that the increase of proteoglycan biosynthesis in sclerotic processes of scleroderma is closely related to an elevated xylosyltransferase activity in blood and demonstrate the validity of xylOSyl transferase as an additional diagnostic marker for determination of sclerosis activity in systemic sclerosis. Expand
Perlecan: an important component of the cartilage pericellular matrix.
TLDR
In vitro studies demonstrate that Pln can trigger chondrogenic differentiation of multipotential mouse CH310T1/2 stem cells as well as maintain the phenotype of adult human chondrocytes and structural mapping indicates that these activities lie entirely within domain I, a region unique to Pln, and that they require glycosaminoglycans. Expand
Chondrogenic Activity of the Heparan Sulfate Proteoglycan Perlecan Maps to the N‐terminal Domain I
  • M. French, R. Gomes, +4 authors D. Carson
  • Biology, Medicine
  • Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
  • 2002
TLDR
This matrix proteoglycan (PG) found at sites of cartilage formation in vivo is likely to enhance early stage differentiation induced by soluble chondrogenic factors. Expand
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