The Fluorescent Toolbox for Assessing Protein Location and Function

  title={The Fluorescent Toolbox for Assessing Protein Location and Function},
  author={B. Giepmans and S. Adams and M. Ellisman and R. Tsien},
  pages={217 - 224}
  • B. Giepmans, S. Adams, +1 author R. Tsien
  • Published 2006
  • Biology, Medicine
  • Science
  • Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small… CONTINUE READING
    2,337 Citations
    A New Approach to Fluorescence Microscopy
    • 5
    • PDF
    Chemical tags: applications in live cell fluorescence imaging.
    • 88
    Advances in chemical labeling of proteins in living cells
    • 24
    A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.
    • 127
    Bright ideas for chemical biology.
    • 797
    • PDF
    A small protein probe for correlated microscopy of endogenous proteins
    • 12
    • PDF


    Fluorescent proteins as a toolkit for in vivo imaging.
    • 444
    • PDF
    Creating new fluorescent probes for cell biology
    • 1,370
    • PDF
    Selective chemical labeling of proteins in living cells.
    • 132
    Fluorescent labels for proteomics and genomics.
    • A. Waggoner
    • Biology, Medicine
    • Current opinion in chemical biology
    • 2006
    • 176
    The green fluorescent protein.
    • R. Tsien
    • Biology, Medicine
    • Annual review of biochemistry
    • 1998
    • 4,367
    • PDF
    Site-specific labeling of proteins with small molecules in live cells.
    • 274
    • PDF
    A guide to choosing fluorescent proteins
    • 2,650
    • PDF
    Imaging protein molecules using FRET and FLIM microscopy.
    • 643