The Fluorescent Toolbox for Assessing Protein Location and Function

  title={The Fluorescent Toolbox for Assessing Protein Location and Function},
  author={Ben N. G. Giepmans and Stephen R. Adams and Mark Ellisman and Roger Tsien},
  pages={217 - 224}
Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small… 
A New Approach to Fluorescence Microscopy
Methods for tagging specific biomolecules with fluorescent labels, such as green fluorescent protein (GFP), provide a toolbox for the observation of protein organization, interactions, and dynamics that enables the visualization of a host of biological phenomena.
Laser Imaging of Functional Molecules in the Living Cell
A brief introduction to fluorescent probes for cell imaging by laser microscopy and methods for tagging of proteins with fluorescent probes are provided and a summary of what biological phenomena the authors can image using fluorescent probes and laser microscopes is summarized.
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