The Fine Structural Localization of Adenosine Triphosphatase in the Small Intestine, Kidney, and Liver of the Rat

@article{Ashworth1963TheFS,
  title={The Fine Structural Localization of Adenosine Triphosphatase in the Small Intestine, Kidney, and Liver of the Rat},
  author={Charles T. Ashworth and F. J. Luibel and S. C. Stewart},
  journal={The Journal of Cell Biology},
  year={1963},
  volume={17},
  pages={1 - 18}
}
The distribution of the reaction product of a staining method for adenosine triphosphatase (ATPase) in rat small intestine, kidney, and liver was studied with electron microscopy. Several procedures were tried but the best results were obtained from tissue that had been quenched in liquid nitrogen, sectioned at 25 micro in a cryostat, fixed for 30 to 90 minutes at 4 degrees C in formalin-sucrose buffered to pH 7.2, incubated with substrate, and then osmicated and prepared for electron… CONTINUE READING
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