The Expression and Regulation of Cholesterol 7α-Hydroxylase

  title={The Expression and Regulation of Cholesterol 7α-Hydroxylase},
  author={John Y. L. Chiang and W. G.D. and Diana Bou Karam and T. PH. and Xiufen Yang and De Peng Wang},
To study the molecular mechanism of regulation of cholesterol 7a-hydroxylase, the rate-limiting enzyme in bile acid synthesis, we have purified a rat liver cholesterol 7ahydroxylase, obtained a specific antibody, cloned the cDNA and expressed an active enzyme in E. Coli. Using these reagents, we studied the regulation of 7a-hydroxylase by cholesterol, oxysterols, bile acids, hormones and other drugs. All results indicate that 7a-hydroxylase is regulated at the gene transcriptional level. The… CONTINUE READING