The Evolution of Three-Dimensional Cell Cultures Towards Unimpeded Regenerative Medicine and Tissue Engineering

@inproceedings{Evangelatov2013TheEO,
  title={The Evolution of Three-Dimensional Cell Cultures Towards Unimpeded Regenerative Medicine and Tissue Engineering},
  author={Aleksandar Evangelatov and Roumen Pankov},
  year={2013}
}
The idea that cellular survival and growth could be maintained outside the body was recog‐ nized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [1]. Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for… 

Figures from this paper

In vitro three-dimensional models in cancer research: a review
TLDR
The 3D tumour models currently available and the more advanced techniques from the tissue-engineering field used to create a more clinically accurate ex vivo tumour model are reviewed and the drastic differences in drug responses between 3D and 2D models are highlighted.
A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications
TLDR
It is demonstrated that endometrium derived from porcine decellularized uteri has potential for in vitroendometrial culture and as treatment for endometrial pathologies, and physicochemical features of the EndoECM are investigated, including gelation kinetics, ultrastructure, and proteomic profile.
Cell proliferation in in vivo‐like three‐dimensional cell culture is regulated by sequestration of ERK1/2 to lipid rafts
TLDR
A native 3D culture of cells is described, based on the GD25β1 fibroblast cell line, and its use for investigating cell proliferation in in vivo‐like conditions is described.
Engineering a tissue mimic for predictive nanoparticle assessment
Bio-scientific research has relied heavily on models of cell monolayers cultured on plastic. For most cell types, this does not represent their in vivo tissue organisation well. As a result they
Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms.
TLDR
The aim is to engineer instructive systems to regulate the spatio-temporal delivery of proper signalling based on the biological mechanisms of the different events that occur in the host microenvironment to create instructive microenvironments for wound healing.
Modeling the lung: Design and development of tissue engineered macro- and micro-physiologic lung models for research use
TLDR
The goal of engineering good 3D human models is the formation of physiologically functional respiratory tissue surrogates which can be used as pathogenesis models or in the case of 2D screening systems for drug therapy evaluation as well as human toxicity testing.
Recent advances in biomaterials for 3D scaffolds: A review
TLDR
A comprehensive summary of recent trends in development of single- (metal, ceramics and polymers), composite-type and cell-laden scaffolds that in addition to mechanical support, promote simultaneous tissue growth, and deliver different molecules or cells with therapeutic or facilitating regeneration effect is offered.
Immune-competent in vitro co-culture models as an approach for skin sensitisation assessment.
TLDR
The technical aspects to take into consideration when co-culturing depending on the desired objective are addressed and the different keratinocytes and dendritic cells co- Cultures developed in 2D and 3D are described.
Critical analysis of 3-D organoid in vitro cell culture models for high-throughput drug candidate toxicity assessments.
TLDR
Issues, challenges, and future directions for the field to improve drug assay predictive power and reliability of 3-D models are reviewed.
Multiscale Analysis of Extracellular Matrix Remodeling in the Failing Heart.
TLDR
The authors' multi-parametric approach has highlighted repercussions of ECM remodeling on cell homing, CF activation and focal adhesion protein expression via hyper-activated YAP signaling during HF.

References

SHOWING 1-10 OF 151 REFERENCES
Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines.
TLDR
A long-term, feeder cell-free culture system was successful on Matrigel in combination with mTeSR1 culture medium, but a xeno-free, fully defined, and reproducible feedercell-free hESC culture method still remains to be developed.
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
TLDR
Methods for determining final cell viability and immunohistological staining to examine the expression and morphology of typical proteins important for contraction and cellular coupling in fibrin hydrogel constructs for tissue engineering are described.
Stroma-derived three-dimensional matrices are necessary and sufficient to promote desmoplastic differentiation of normal fibroblasts.
TLDR
It is demonstrated that in vivo-like three-dimensional matrices appear to have the necessary topographical and molecular information sufficient to induce desmoplastic stroma differentiation of normal fibroblasts.
Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes.
TLDR
Phenotypic analysis showed that chondrocytes, when three-dimensionally cultured within the HA matrix, expressed transcripts encoding collagen type II and aggrecan, and produced sulfated glycosaminoglycans (s-GAG), indicating chondrogenic differentiation, and the new HA matrix appears as a potentially promising scaffold for the three-dimensional culture of chondROcytes for cartilage tissue engineering.
Tissue specific synthetic ECM hydrogels for 3-D in vitro maintenance of hepatocyte function.
TLDR
Results indicate that customized HA hydrogels with liver-specific ECM components may be an efficient method for expansion human hepatocytes in vitro for cell therapy and drug and toxicology screening purposes.
Mechano-functional assessment of human mesenchymal stem cells grown in three-dimensional hyaluronan-based scaffolds for cartilage tissue engineering.
TLDR
The results demonstrate the competency of the neo-formed cartilage-like tissue in relation to its mechanical and cellular properties, and the importance, for future clinical use, of implanting this construct after 14 days of culture.
Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels.
TLDR
PLECM represents a favorable substrate for in vitro culture of human hepatocytes and is compared to Matrigel, which has been shown to be the most useful matrix for static, traditional hepatocyte culture.
Feeder-free growth of undifferentiated human embryonic stem cells
TLDR
A successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least 130 population doublings and are suitable for scaleup production is demonstrated.
Tissue engineering of lung: the effect of extracellular matrix on the differentiation of embryonic stem cells to pneumocytes.
TLDR
It is concluded that the choice of the coating protein can greatly affect the differentiation of ESCs, and laminin-332-coated PDLLA provided an ECM-degradable scaffold combination that is suitable for engineering of lung tissue constructs.
Biomechanical and biochemical characteristics of a human fibroblast-produced and remodeled matrix.
TLDR
As *CDM is produced by human cells in a chemically defined culture medium and is mechanically robust, it may be a viable living tissue equivalent for many connective tissue replacement applications requiring initial mechanical stability yet a high degree of biocompatibility.
...
1
2
3
4
5
...