The Efficient Gene Delivery into Human Mesenchymal Stem Cells Using Retroviral Vectors

@inproceedings{Kim2004TheEG,
  title={The Efficient Gene Delivery into Human Mesenchymal Stem Cells Using Retroviral Vectors},
  author={Sung Soo Kim and Bum Jun Kim and Haeyoung Suh and H Kim},
  year={2004}
}
Human mesenchymal stem cells (hMSCs) are multipotent stem cells that can differentiate into several mesenchymal lineage cells. In this study, we established an efficient method for gene delivery into these cells. Non-viral transfection reagents that were commercially available yielded 5% efficiency. In contrast, a retroviral vector yielded more than 46% transduction, which was further increased to 90% by repetitive infection. Retroviral transduction did not alter the multipotency of hMSCs. Thus… CONTINUE READING