• Corpus ID: 5875173

The Efficacy of CD 3 x CD 19 Bispecific Monoclonal Antibody ( BsAb ) in a Clonogenic Assay : The Effect of Repeated Addition of BsAb and Interleukin-2

  title={The Efficacy of CD 3 x CD 19 Bispecific Monoclonal Antibody ( BsAb ) in a Clonogenic Assay : The Effect of Repeated Addition of BsAb and Interleukin-2},
  author={Anette J. G. Geerars and Ba De and Bert J.E.G. Bast and Bert and D R Gast},
To evaluate the potency by which human T cells are targeted and activated by bispecific monoclonal antibodies (BsAbs) t o lyse tumor cells, a clonogenic assay was developed. The efficacy of a CD3xCD19 BsAb binding to both the CD3 T-cell antigen and the CD19 B-cell antigen was already proven in "Cr-release assays and in 3-day activation cultures. To achieve more quantitative results, a 14-day clonogenic assay, based on limiting-dilution, was performed for the determination of the initial and… 
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