Paramylon (β-1,3-glucan) content in wild type and WZSL mutant of Euglena gracilis. Effects of growth conditions
Two studies were conducted to determine the effects of two different feed additives on the performance of laying hens. Study 1 examined the effects of non-starch polysaccharide degrading enzyme inclusion in a low energy diet. Study 2 examined the effects of supplementing a paramylon zinc polysaccharide complex at increasing concentrations. Both studies utilized a randomized complete block design for treatment assignment, and data were analyzed using the Glimmix procedure in SAS 9.4 for windows. Each phase tested a positive control, negative control with lower ME and nutrient density, and two different xylanases as supplements to the negative control diet. There were 72 cages with 3 Bovan White Leghorns per cage used for this study. Data for percent egg production, feed intake, bodyweight, egg mass, and feed conversion were collected. Significant differences were seen for all parameters (p≤0.05) except egg production (phase 1: p=0.47; phase 2: p=0.54) and baseline bodyweight (p=0.63). The enzymes did not improve the performance of the birds fed the low ME negative control diet up to the level of those fed the positive control. Hy-Line Brown Leghorn hens per cage. There were 4 treatments tested, including a control and three diets with increasing dosage of Algamune TM ZPC (at 100, 200, and 300 g/ton), a paramylon zinc polysaccharide complex. Recorded parameters included percent were no significant differences among treatments (p≤0.05), however egg omega-3 fatty acid content was approaching significance comparing the low and high levels of paramylon supplementation (p=0.0688). The Algamune TM ZPC did not negatively affect performance and may be considered safe to supplement as a source of omega-3 and Zn to poultry at the levels tested in this study. Hanford for reviewing and editing my thesis. They have provided great insight and helped me to learn how to be a better scientist and writer. Of course I still have much to learn! I also need to thank my fellow students and past graduates in the poultry program, as well as my mom, dad, sister, and brother. Everyone has been so supportive throughout my masters program and I look forward to working on my PhD!