The Effects of Methylglyoxal on Glutathione S-Transferase from Nicotiana tabacum

  title={The Effects of Methylglyoxal on Glutathione S-Transferase from Nicotiana tabacum},
  author={Md. Anamul Hoque and Misugi Uraji and Mst. Nasrin Akhter Banu and Izumi C. Mori and Yoshimasa Nakamura and Yoshiyuki Murata},
  journal={Bioscience, Biotechnology, and Biochemistry},
  pages={2124 - 2126}
Methylglyoxal (MG) is one of the aldehydes that accumulate in plants under environmental stress. Glutathione S-transferases (GSTs) play important roles, including detoxification, in the stress tolerance systems of plants. To determine the effects of MG, we characterized recombinant GST. MG decreased GST activity and thiol contents with increasing K m. GST can serve as a target of MG modification, which is suppressed by application of reduced glutathione. 

Methylglyoxal inhibition of cytosolic ascorbate peroxidase from Nicotiana tabacum

The present study suggests that inhibition of APX is in part due to the modification of amino acids by MG, which resulted in a reduction ofAPX activity and modifications of amino groups in rNtcAPX with increasing Km for ascorbate.

Glyoxalase System: A Glutathione-Dependent Pathway for Abiotic Stress Tolerance in Plants

Light is shed on the dependence of the glyoxalase pathway on GSH as the key player in regulating the pathway and in maintaining a higher “reduced to oxidized” GSH ratio.

Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

MG is now considered as a potential biochemical marker for plant abiotic stress tolerance, and is receiving considerable attention by the scientific community, and the concept of MG signaling is evaluated.

Methylglyoxal induces inhibition of growth, accumulation of anthocyanin, and activation of glyoxalase I and II in Arabidopsis thaliana

MG can reduce plant growth as a toxic molecule and can stimulate stress responses as a signal molecule under stress conditions.

Coordinated Actions of Glyoxalase and Antioxidant Defense Systems in Conferring Abiotic Stress Tolerance in Plants

A novel glyoxalase enzyme has been detected in plants, providing a shorter pathway for MG detoxification, which is also a signpost in the research of abiotic stress tolerance.

Methylglyoxal, Triose Phosphate Isomerase, and Glyoxalase Pathway: Implications in Abiotic Stress and Signaling in Plants

An insight is provided to the role of MG and glyoxalases in plant stress response with special mention about the possible involvement of MG in signaling pathway.

The Glyoxalase System: A Possible Target for Production of Salinity-Tolerant Crop Plants

This chapter summarizes the current knowledge and understanding of MG and the glyoxalase pathway, with respect to salinity stress tolerance and the potential for use of genetic engineering of gly oxalase genes into crop plants to improve crop yields under salt stress.

Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification

This review examines the structure and the stress regulation of the pathways involved in glutathione synthesis and degradation, and presents recent developments on the organization of the glyoxalase pathway in which alternative splicing generate a number of isoforms targeted to various subcellular compartments.

Overexpression of ThGSTZ1 from Tamarix hispida improves tolerance to exogenous ABA and methyl viologen

Key messageMolecular analysis of a zeta subfamilyGSTgene fromT. hispidainvolved in ABA and methyl viologen tolerance in transgenic Arabidopsis andTamarix.AbstractGlutathione S-transferase (GST) genes

Plant glutathione transferases

The soluble glutathione transferases (GSTs) represent an excellent example of how protein families can diversify to fulfill multiple functions while conserving form and structure.

parB: an auxin-regulated gene encoding glutathione S-transferase.

    Y. TakahashiT. Nagata
    Proceedings of the National Academy of Sciences of the United States of America
  • 1992
The role of parB is discussed in relation to the induction of proliferative activity in differentiated and nondividing mesophyll protoplasts of tobacco as well as detoxification of xenobiotics in plant and animal cells.

Effects of MG on Km Values for rNtGST Using GSH and CDNB Treatment Km (mM) GSH CDNB Control

    XR, Biochimie,
  • 2005