The Effects of Gap Junction Blockage on Neuronal Differentiation of Human NTera2/Clone D1 Cells

  title={The Effects of Gap Junction Blockage on Neuronal Differentiation of Human NTera2/Clone D1 Cells},
  author={Mahmud Bani‐Yaghoub and John F. Bechberger and T. Michael Underhill and Christian C. G. Naus},
  journal={Experimental Neurology},
Gap junctions are intercellular channels which provide for the passage of small ions and molecules (MW <1200 D) among adjacent cells. The NTera2/clone D1 (NT2/D1) cells are CNS precursors which differentiate into NT2-N neurons upon treatment with retinoic acid (RA) and antiproliferative agents. In this study, the effects of gap junction blockers 18 alpha-glycyrrhetinic acid (GRA) and carbenoxolone (CBX) have been compared with those of oleanolic acid (OLA) and glycyrrhizic acid (GZA), GRA… 

Stage-dependent effects of cell-to-cell connections on in vitro induced neurogenesis.

The results indicate that NE-4C cells provide a useful model system to investigate the role of contact communication during some early steps of neurogenesis, and the contact interactions formed between already committed progenitor cells seemed to hinder the formation of novel neurons.

Blocked Gap Junctional Coupling Increases Glutamate‐Induced Neurotoxicity in Neuron‐Astrocyte Co‐Cultures

The hypothesis that gap junctions play a neuroprotective role against glutamate cytotoxicity is supported, as blocking agents did not affect astrocytic uptake of glutamate.

The Carboxyl-terminal Domain of Connexin43 Is a Negative Modulator of Neuronal Differentiation*

In situ analysis of embryonic day 16 brains revealed increased β-III-tubulin expression in germinal zones of Cx43-null compared with that of WT littermates, indicating that Cx 43, and specifically its carboxyl terminus, is crucial for signaling mechanisms preventing premature neuronal differentiation during embryonic brain development.

The role of connexins in the differentiation of NT2 cells in Sertoli-NT2 cell tissue constructs grown in the rotating wall bioreactor

It is reported here that the gap junction protein connexin 43 is decreased among differentiated NT2N neurons and is important in the differentiation of NT2 cells in the SNAC tissue construct.

Bimodal inhibition of connexin 43 gap junctions decreases ACTH-induced steroidogenesis and increases bovine adrenal cell population growth.

The decreased responsiveness and increased number of cells in the population after gap junction function was decreased by either GA treatment or antisense transfection, suggests that gap junctions may be necessary factors in ACTH-stimulated responsiveness and growth control in the adrenal gland.

All-Trans Retinoic Acid Regulates Cx43 Expression, Gap Junction Communication and Differentiation in Primary Lens Epithelial Cells

Treatment with ATRA significantly increased Cx43 expression and GJIC in canine LEC, and these effects were associated with increased LEC differentiation.

Neuronal differentiation requires a biphasic modulation of gap junctional intercellular communication caused by dynamic changes of connexin43 expression

A dual function of Cx43 and GJIC in the neural development of ReNcell VM197 human progenitor cells is suggested, necessary to maintain cells in a proliferative state and to complete neuronal differentiation, including the establishment of a neural network.

Enhanced Neurite Outgrowth in PC12 Cells Mediated by Connexin Hemichannels and ATP*

The results of the current study suggest that connexins may play an important role in neuronal differentiation by non-traditional mechanisms.

The Effects of Bone Morphogenetic Protein 2 and 4 (BMP2 and BMP4) on Gap Junctions during Neurodevelopment

Data suggest that BMPs may play a critical role in morphogenesis of P19 cells while they affect gap junctions, and that cells treated with BMP2 or BMP4 lose their capacity to differentiate into neurons but not astrocytes, while they maintain extensive gap junctional communication.

Apoptosis in cultured hNT neurons.




Reduction of connexin43 expression and dye‐coupling during neuronal differentiation of human NTera2/clone D1 cells

It is concluded that both connexin43 expression and dye coupling capacity decrease during neuronal differentiation of NT2/D1 cells, confirming the functionality of gap junctions in these cells.

Blocking gap junctional intercellular communication in myoblasts inhibits myogenin and MRF4 expression.

The results suggest that intercellular communication via gap junctions plays an important role in skeletal muscle development and perhaps in the cell signaling events that trigger the activation of muscle-specific MRF genes.

Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons

  • SJ PleasureC. PageV. Lee
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1992
It is concluded that NT2 cells and NT2-N cells represent a unique model system for studies of human neurons, and a novel vehicle for the expression of diverse gene products in terminally differentiated polarized neurons.

Differentiation of NTERA-2 clonal human embryonal carcinoma cells into neurons involves the induction of all three neurofilament proteins

  • V. LeeP. Andrews
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1986
The number of neurofilament- positive cells increased with time after initial exposure to retinoic acid, and although 95% of these cells contained cytokeratin initially, less than 5% of the neuro Filament-positive cells retained cytokersatin 2 weeks later, suggesting that many of the cells expressing all three neurofilaments but no cytokerati exhibited a neuronal morphology.

NTera 2 Cells: A human cell line which displays characteristics expected of a human committed neuronal progenitor cell

It is proposed that NT2/D1 cells are a committed human neuronal precursor cell line which retains some stem cell characteristics and is capable only of terminal differentiation into neurons.

Is intercellular communication via gap junctions required for myoblast fusion?

Observations suggest that intercellular communication through gap junctions might be a necessary step for myoblast fusion.

Transfection of C6 glioma cells with connexin 43 cDNA: analysis of expression, intercellular coupling, and cell proliferation.

Investigation of the role of gap junctions in the tumor characteristics of C6 glioma cells using Lipofectin-mediated transfection to introduce a full-length cDNA encoding connexin 43 revealed an increase in the amount of con Nexin 43 immunoreactivity in the transfected cells, being localized at areas of intercellular contact as well as in the cytoplasm.

Differentiation of hippocampal progenitor cells in vitro: temporal expression of intercellular coupling and voltage- and ligand-gated responses.

The results indicate that certain cytokines may orchestrate the progressive expression of functional neuronal phenotypes in vitro, in which the gradual disappearance of intercellular coupling parallels the onset of voltage-dependent responses and both of which precede the expression of neurotransmitter chemosensitivity.

Switch in gap junction protein expression is associated with selective changes in junctional permeability during keratinocyte differentiation.

A switch in gap junction protein expression in differentiating keratinocytes is accompanied by selective changes in junctional permeability that may play an important role in the coordinate control of the differentiation process.