The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens
@article{Hansen1998TheEO, title={The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens}, author={L. Hansen and Steen Knudsen and S. S{\o}rensen}, journal={Current Microbiology}, year={1998}, volume={36}, pages={341-347} }
Abstract. The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-β-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with newly constructed strains containing inserts without the lacY gene (lacIPOZ). The lactose operon inserts were introduced as single-copy chromosomal inserts to eliminate differences in expression caused by… CONTINUE READING
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