The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens

  title={The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens},
  author={Lars Hestbjerg Hansen and Steen Knudsen and S{\o}ren Johannes S{\o}rensen},
  journal={Current Microbiology},
Abstract. The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-β-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with newly constructed strains containing inserts without the lacY gene (lacIPOZ). The lactose operon inserts were introduced as single-copy chromosomal inserts to eliminate differences in expression caused by… 
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