ALT and AST are two important enzymes in liver that high level of them indicates liver damage. There are some factors that cause increase in these enzymes in blood such as drugs or diseases. Camphor as a herbal medicine is used for its effects on reproductive system, kidney, liver, aborting etc. In this research we have examined the effect of camphor on concentration of enzymes in liver. 40 female rats with average weight 180 ± 20 gram and age 80-90 days are used. They are divided into 5 groups randomly. The control group received no injection. The sham group received olive oil as camphor solvent. The experimental groups 1, 2 and 3 received camphor solution in doses of 25, 50 and 100 mg/kg. Daily injection was done intraperitioneally and amount 0.2 cc. All of them received drug for 14 days. At the end of the injections a sample of serum was taken and was examined by Eliza method in order to determine the concentration of enzymes. The results showed no significant difference between control group and examined groups. Although there were no significant differences but it showed an increase in amount of enzymes in experimental groups compared to control. Maybe by using camphor in higher doses it leads to significant increase in concentration of enzymes. So it suggests that camphor has an stimulating effect on liver enzymes. Keywords-453 camphor; liver; enzyme; rat.