The Effect of Antifreeze Glycoproteins on Survival of Fish Spermatozoa under the Conditions of Long-Term Storage at 4°C

Abstract

It was already shown that antifreeze glycoproteins isolated from the blood of fish occurring in circumpolar regions inhibited the growth of ice crystals both in vitro and in vivo. When the spermatozoa were frozen to liquid nitrogen temperature, addition of antifreeze glycoproteins to cryoconserving media made it possible to decrease twofold the… (More)
DOI: 10.1023/A:1013298003947

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