The Effect of β-Turn Structure on the Passive Diffusion of Peptides Across Caco-2 Cell Monolayers

@article{Knipp1997TheEO,
  title={The Effect of β-Turn Structure on the Passive Diffusion of Peptides Across Caco-2 Cell Monolayers},
  author={Gregory T. Knipp and David G. Vander Velde and Teruna J. Siahaan and Ronald T. Borchardt},
  journal={Pharmaceutical Research},
  year={1997},
  volume={14},
  pages={1332-1340}
}
Purpose. To investigate the relationships between the β-turn structure of a peptide and its passive diffusion across Caco-2 cell monolayers, an in vitro model of the intestinal mucosa. Methods. Linear hydrophilic peptides (Ac-TyrProXaaZaaVal-NH2; Xaa = Gly, Ile and Zaa = Asp, Asn) and hydrophobic (Ac-YaaPro-XaaIleVal-NH2; Yaa = Tyr, Phe and Xaa = Gly, Ile: and Ac-PhePro-XaaIle-NH2; Xaa = Gly, He) peptides were synthesized and their effective permeability coefficients (Peff) were determined… CONTINUE READING