The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

@article{Suri1984TheER,
  title={The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.},
  author={B S Suri and John C. W. Shepherd and Thomas A. Bickle},
  journal={The EMBO journal},
  year={1984},
  volume={3 3},
  pages={575-9}
}
The EcoA restriction enzyme from Escherichia coli 15T- has been isolated. It proves to be an unusual enzyme, clearly related functionally to the classical type I restriction enzymes. The basic enzyme is a two subunit modification methylase. Another protein species can be purified which by itself has no enzymatic activities but which converts the modification methylase to an ATP and S-adenosylmethionine-dependent restriction endonuclease. The DNA recognition sequence of EcoA has an overall… CONTINUE READING