The Early Pathogenesis of Foot-and-Mouth Disease in Cattle After Aerosol Inoculation

@article{Arzt2010TheEP,
  title={The Early Pathogenesis of Foot-and-Mouth Disease in Cattle After Aerosol Inoculation},
  author={Jonathan Arzt and Juan M. Pacheco and Luis L. Rodriguez},
  journal={Veterinary Pathology},
  year={2010},
  volume={47},
  pages={1048 - 1063}
}
To characterize the early events of foot-and-mouth disease virus (FMDV) infection in cattle subsequent to simulated natural exposure, 16 steers were aerosol inoculated with FMDV and euthanized at various times. Samples were collected from each steer antemortem (serum, nasal swabs, and oral swabs) and postmortem (up to 40 tissues per animal) and screened for FMDV by virus isolation and for FMDV RNA by real-time reverse transcription polymerase chain reaction. Tissues that tested positive for… 
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After nasopharyngeal infection, foot-and-mouth disease virus serotype A RNA is shed in bovine milk without associated mastitis
TLDR
The time course of FMD virus (FMDV) excretion in the milk is described and vesiculopustular lesions in the oral and epidermal specimens are characterize and the mammary glands did not have unequivocal evidence of F MDV-induced inflammation.
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TLDR
The overall conclusion is that domestic pigs are unlikely to be competent long-term carriers of infectious F MDV; however, transient persistence of FMDV protein and RNA in lymphoid tissues is common following clinical or subclinical infection.
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References

SHOWING 1-10 OF 34 REFERENCES
Early events in the pathogenesis of foot-and-mouth disease in cattle after controlled aerosol exposure.
TLDR
This aerosol infection method is highly reproducible regarding inoculum dose and volume, and allowed the detailed study of early events in FMDV-infected cattle.
A pathogenesis study of foot-and-mouth disease in cattle, using in situ hybridization.
TLDR
By this technique large amounts of viral nucleic acid were found in coronary band, interdigital cleft and tongue as early as six hours postexposure, indicating a very rapid delivery from the portal of entry to the predilection sites for lesion development.
Pathogenesis of wild-type and leaderless foot-and-mouth disease virus in cattle
TLDR
It appears that after aerosol exposure to A12-IC, early replication is in the region of the lung, with subsequent dissemination to distal sites, compared with the A 12-LLV2 virus, which is much less widely disseminated in the lung at 24 hpe, with no lesions or virus detectable in secondary sites at 72 hpe.
Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues.
TLDR
Viral RNA was detected by in situ hybridisation within cells of the soft palate, tonsil and pharynx up to 17 days post-infection and may assist in identifying specific sites of virus persistence.
Pathogenesis of foot-and-mouth disease: the lung as an additional portal of entry of the virus.
TLDR
It is confirmed that FMD virus can enter the bloodstream via the lung, followed by haematogenic infection of the pharynx and other replication sites simultaneously.
Pathogenesis of foot-and-mouth disease in swine, studied by in-situ hybridization.
TLDR
With this technique, foot-and-mouth disease virus was shown to be widely disseminated in all epidermal tissues, regardless of histologically apparent cellular disruption.
The pathogenesis of natural and simulated natural foot-and-mouth disease infection in cattle.
TLDR
The distribution and amounts of virus in the tissues of 23 cattle killed before the onset of viraemia indicated that the pharyngeal area was the most likely site of initial infection and virus growth.
Airborne excretion of foot-and-mouth disease virus.
TLDR
Under conditions of relative humidity greater than 70% and at low temperatures, survival of virus to a distance of 100 km is likely to occur and because of the minute respiratory volume the aerosol would be sampled more efficiently by cattle than pigs or sheep and by large animals than by small.
Optimization of Immunohistochemical and Fluorescent Antibody Techniques for Localization of Foot-and-Mouth Disease Virus in Animal Tissues
  • J. Arzt, D. Gregg, A. Clavijo, L. Rodriguez
  • Biology, Medicine
    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 2009
TLDR
Immunohistochemical and fluorescent antibody techniques were optimized for the detection of Foot-and-mouth disease virus structural and nonstructural proteins in frozen and paraformaldehyde-fixed, paraffin-embedded tissues of bovine and porcine origin and cells at the leading edge of vesicles were positive for FMDV antigens.
Analysis of foot-and-mouth disease virus integrin receptor expression in tissues from naïve and infected cattle.
TLDR
At the cellular level the expression of these two integrins correlates with susceptibility to infection and may contribute substantially to viral tropism in FMD pathogenesis.
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