The ETSI Transcription Factor Is Expressed during Epithelial- Mesenchymal Transitions in the Chick Embryo and Is Activated in Scatter Factor-stimulated

Abstract

In embryos and in human tumors, the expression of the ETSI transcription factor correlates with the occurrence of invasive processes. Although this was demonstrated in cells of mesodermal origin, the expression of ETSI was not detected in epithelial cells. In the present study, we show that during early organogenesis in the chick embryo, ETSI mRNA expression was transiently induced in epithelial structures, during emigration of neural crest cells and dispersion of somites into the mesenchymal sclerotome. In contrast, the expression of ETSI was not detected in situations where epithelial layers stayed cohesive while forming a new structure, such as the dermomyotome forming the myotome. The involvement of ETSI in epithelial cell dissociation was examined in MDCK epithelial cells stimulated by scatter factor/hepatocyte growth factor (SF/HGF), a potent inducer of cell dissociation and motility. SFIHGF was found to stimulate ETSI mRNA and protein expressions, and these increases coincided with the dispersion of cells and the expression of protease mRNAs, such as urokinase-type plasminogen activator and collagenase, but not with the protease inhibitor, plasminogen activator inhibitor type I . Furthermore, we showed that SFIHGF was able to induce a transcriptional response involving ETSI by using artificial as well as cellular promoters, such as the Received 8/20/96; revised 3/13/97; accepted 3/24/97. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to mdicate this fact. 1 This work was supported by the Institut Pasteur de Lille and the Centre National de Ia Recherche Scientifique, and by grants from the Association pour Ia Aecherche contre Ic Cancer, Ugue Nationale contre Ic Cancer and Groupernent Fran#{231}aisdes Entreprises pour Ia Lutte contre Ic Cancer. 2 To whom requests for reprints should be addressed. Phone: (33) 20.87.10.91 ; Fax: (33) 20.87.1 1 .1 1 ; E-mail: vfafeur@infobiogen.fr. 3 Present address: Fred Hutchinson Cancer Research Center, Seattle, WA 98104. urokinase-type plasminogen activator and collagenase I promoters, containing RAS-responsive elements with essential ETS-binding sites. These data demonstrate expression of ETSI during epithelial-mesenchymal transitions in the developing embryo and show that ETSI can act as a downstream effector of SF/HGF in MDCK epithelial cells. Taken together, these data identify ETS1 as a molecular actor of epithelial cell dissociation.

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@inproceedings{Tulasne2005TheET, title={The ETSI Transcription Factor Is Expressed during Epithelial- Mesenchymal Transitions in the Chick Embryo and Is Activated in Scatter Factor-stimulated}, author={David Tulasne and Chnstophe Qu233va and Chantal Vercamer and Virginie Mattot and Dominique St233helin and Xavier Desbiens and Bernard Vandenbunder}, year={2005} }