The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins

  title={The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins},
  author={Camilla Raiborg and Harald Stenmark},
Selective trafficking of membrane proteins to lysosomes for destruction is required for proper cell signalling and metabolism. Ubiquitylation aids this process by specifying which proteins should be transported to the lysosome lumen by the multivesicular endosome pathway. The endosomal sorting complex required for transport (ESCRT) machinery sorts cargo labelled with ubiquitin into invaginations of endosome membranes. Then, through a highly conserved mechanism also used in cytokinesis and viral… 
ESCRT-dependent cargo sorting at multivesicular endosomes.
ESCRT and Membrane Protein Ubiquitination.
This chapter briefly discusses membrane protein ubiquitination, endocytosis, and summarize current knowledge on the ESCRT machinery in the MVB pathway.
Coats of endosomal protein sorting: retromer and ESCRT.
The ESCRT machinery: From the plasma membrane to endosomes and back again
This review focuses on the molecular mechanisms by which the ESCRT proteins facilitate cargo sequestration and membrane remodeling and highlights their unique roles in cellular homeostasis.
How Ubiquitin Functions with ESCRTs
The relationship between ESCRTs and Ub in MVB sorting and viral budding is explored and hints that both ESCRT and Ub work together in the processes such as cytokinesis, transcription and autophagy are beginning to emerge.
Membrane fission reactions of the mammalian ESCRT pathway.
The endosomal sorting complexes required for transport (ESCRT) pathway was initially defined in yeast genetic screens that identified the factors necessary to sort membrane proteins into intraluminal
Vesicle formation within endosomes: An ESCRT marks the spot
Recent findings regarding the structure and function of the ESCRT machinery are discussed, highlighting specific roles for ESCRT-0 and ESCRT -III in regulating cargo selection and vesicle formation.
Cell biology of the ESCRT machinery.
Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles.
This chapter describes a detailed protocol for studying vacuolar sorting and degradation of PIN2-GFP by using T-DNA insertional mutants, dexamethasone (DEX) inducible RNAi lines, and other tools, including Fei-Mao (FM) dye staining and dark treatment.


ESCRT complexes and the biogenesis of multivesicular bodies.
  • J. Hurley
  • Biology
    Current opinion in cell biology
  • 2008
The emerging shape of the ESCRT machinery
Several previously unsuspected key links between multiple ESCRT subunits, phospholipids and ubiquitin have now been elucidated, which, together with the detailed morphological analyses of ESCRT-depletion phenotypes, provide new insights into the mechanism of MVB biogenesis.
Receptor downregulation and multivesicular-body sorting
The sorting of proteins into the inner vesicles of multivesicular bodies is required for many key cellular processes, which range from the downregulation of activated signalling receptors to the
Structure of the ESCRT-II endosomal trafficking complex
The crystal structure of the core of the yeast ESCRT-II complex, which contains one molecule of the Vps protein Vps22, the carboxy-terminal domain of VPS36 and two molecules of Vps25, and has the shape of a capital letter ‘Y’ is reported.
Vps27 recruits ESCRT machinery to endosomes during MVB sorting
This work proposes that compartmental specificity for the MVB sorting reaction is the result of interactions of Vps27 with phosphatidylinositol 3-phosphate and ubiquitin, and recruits/activates ESCRT-I on endosomes, thereby facilitating sorting of ubiquitinated MVB cargoes.
Hrs and SNX3 Functions in Sorting and Membrane Invagination within Multivesicular Bodies
It is reported that Hrs is essential for lysosomal targeting but dispensable for multivesicular body biogenesis and transport to late endosomes, and it is found that the PtdIns3P-binding protein SNX3 is required for multiveicular body formation, but not for EGF receptor degradation.
Evolution of the Multivesicular Body ESCRT Machinery; Retention Across the Eukaryotic Lineage
The ESCRT system is an ancient and well‐conserved feature of eukaryotic cells but with key differences between diverse lineages, as confirmed by direct study in trypanosomes.