The Dynamin 2 inhibitor Dynasore affects the actin filament distribution during mouse early embryo development

Abstract

Dynamin 2 is a large GTPase notably involved in clathrin-mediated endocytosis, cell migration and cytokinesis in mitosis. Our previous study identified that Dynamin 2 regulated polar body extrusion in mammalian oocytes, but its roles in early embryo development, remain elusive. Here, we report the critical roles of Dynamin 2 in mouse early embryo development. Dynamin 2 accumulated at the periphery of the blastomere during embryonic development. When Dynamin 2 activity was inhibited by Dynasore, embryos failed to cleave to the 2-cell or 4-cell stage. Moreover, the actin filament distribution and relative amount were aberrant in the treatment group. Similar results were observed when embryos were cultured with Dynasore at the 8-cell stage; the embryos failed to undergo compaction and develop to the morula stage, indicating a role of Dynamin 2 in embryo cytokinesis. Therefore, our data indicate that Dynamin 2 might participate in the early embryonic development through an actin-based cytokinesis.

DOI: 10.1262/jrd.2014-079

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@inproceedings{Wang2015TheD2, title={The Dynamin 2 inhibitor Dynasore affects the actin filament distribution during mouse early embryo development}, author={Qiao-Chu Wang and Jun Liu and Xing Duan and Xiang-Shun Cui and Nam-Hyung Kim and Bo Xiong and Shao-Chen Sun}, booktitle={The Journal of reproduction and development}, year={2015} }