The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway.

@article{Schierle2003TheDS,
  title={The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway.},
  author={Clark Friedrich Schierle and Mehmet Berkmen and Damon Huber and Carol A. Kumamoto and D. Boyd and Jon et Beckwith},
  journal={Journal of bacteriology},
  year={2003},
  volume={185 19},
  pages={5706-13}
}
The Escherichia coli cytoplasmic protein thioredoxin 1 can be efficiently exported to the periplasmic space by the signal sequence of the DsbA protein (DsbAss) but not by the signal sequence of alkaline phosphatase (PhoA) or maltose binding protein (MBP). Using mutations of the signal recognition particle (SRP) pathway, we found that DsbAss directs thioredoxin 1 to the SRP export pathway. When DsbAss is fused to MBP, MBP also is directed to the SRP pathway. We show directly that the DsbAss… CONTINUE READING
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Molecular cloning: a laboratory manual, 3rd ed

  • J. Sambrook, D. W. Russell
  • 2001
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