The Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain.

@article{Gauger1993TheDK,
  title={The Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain.},
  author={A. Gauger and Lawrence S B Goldstein},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 18},
  pages={
          13657-66
        }
}
Kinesin light chain (KLC) complexes with the kinesin heavy chain (KHC) to form native kinesin. Proposed functions of KLC include coupling of cargo to KHC or modulation of KHC ATPase activity. In this paper we use the KHC tail, which binds specifically to KLC in blot overlays, as a probe to clone a cDNA encoding KLC from a Drosophila expression library. The identified clone encodes a protein with 70% amino acid identity to rat KLC. Drosophila KLC is predicted to form an alpha-helical coiled-coil… Expand
Kinesin Light-Chain KLC3 Expression in Testis Is Restricted to Spermatids1
TLDR
The characterization of the KLC3 gene suggests that it carries out a unique and specialized role in spermatids, and may be involved in cargo binding and regulation of kinesin activity. Expand
Association of Kinesin Light Chain with Outer Dense Fibers in a Microtubule-independent Fashion*
TLDR
It is suggested that KLC3 could play a microtubule-independent role during formation of sperm tails and identify and characterize a novel interaction between a KLC and a non-microtubule macromolecular structure. Expand
Defective Kinesin Heavy Chain Behavior in Mouse Kinesin Light Chain Mutants
TLDR
Biochemical analyses demonstrated that KLC1 mutant mice have a pool of KIF5A not associated with any known KLC subunit, which best support models that suggest that K LC1 is essential for proper KHC activation or targeting. Expand
Kinesin Light Chains Are Essential for Axonal Transport in Drosophila
TLDR
The similarity of the Klc and Khc mutant phenotypes indicates that KLC is essential for kinesin function, perhaps by tethering KHC to intracellular cargos or by activating the kinesIn motor. Expand
A specific light chain of kinesin associates with mitochondria in cultured cells.
TLDR
Data indicate a specific association of a particular KLC (B type) with mitochondria, revealing that different KLC isoforms can target kinesin to different cargoes. Expand
The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline
TLDR
It is shown that two unexpected highly conserved domains, namely the auto-inhibitory IAK and the auxiliary microtubule-binding motifs, are crucial for transport by Kinesin-1 and that, although not all cargos are conserved, their transport involves the most Conserved domains of animal KHCs. Expand
Vaccinia Protein F12 Has Structural Similarity to Kinesin Light Chain and Contains a Motor Binding Motif Required for Virion Export
TLDR
The report of a viral KLC-like protein containing a KBS that is conserved in several cellular proteins advances the understanding of how VACV recruits the kinesin motor to virions, and exemplifies how viruses use molecular mimicry of cellular components to their advantage. Expand
Unique and Shared Functions of Kinesin Light Chain and Milton in Drosophila Oogenesis and Axonal Transport
TLDR
The data suggest that Klc is working as a critical factor in these Kinesin-1 driven transport events, including a previously undescribed role in mitochondrial transport. Expand
The ribosome receptor, p180, interacts with kinesin heavy chain, KIF5B.
TLDR
A yeast two-hybrid assay was employed to identify additional binding partners of the kinesin heavy chain isoform KIF5B and identified the ribosome receptor, p180, as a Kif5B-binding protein. Expand
Two Kinesin Light Chain Genes in Mice
TLDR
The results indicate that the mammalian nervous system contains multiple kinesin light chain gene products with potentially distinct functions. Expand
...
1
2
3
4
5
...