The Decrease of Extracted Apoplast Protein in Soybean Root Tip by Aluminium Treatment

Abstract

Aluminium effect on the mobility of apoplast protein in root tips was studied. Two-day seedlings of soybean (Glycine max. (L.) Merr. cv. Tsurunoko) were treated with 50 μM AlCl3 for 2 h. Using infiltration method, the apoplast protein in root tips was extracted with 20 or 100 mM MgCl2. When 20 mM MgCl2 was used to collect weakly bound protein to apoplast, the amount of protein extracted was reduced to be about 20 % compared with that of control and the band of 97 kDa disappeared in SDS-PAGE gel. However, the 97 kDa protein could be extracted by 100 mM MgCl2, which were used for extraction of more tightly bound protein to apoplast, and the amount was estimated to be the same as that of control. When the protein was further developed in two-dimensional electrophoresis, three spots were found between pI 6.4 and 6.5. This is the first report of an Al effect on the mobility of apoplast protein.

DOI: 10.1023/A:1024302924984

Cite this paper

@article{Kataoka2004TheDO, title={The Decrease of Extracted Apoplast Protein in Soybean Root Tip by Aluminium Treatment}, author={Tomomori Kataoka and Jun-ichi Furukawa and Tomoko M. Nakanishi}, journal={Biologia Plantarum}, year={2004}, volume={46}, pages={445-449} }