The DXD motif is required for GM2 synthase activity but is not critical for nucleotide binding.

@article{Li2001TheDM,
  title={The DXD motif is required for GM2 synthase activity but is not critical for nucleotide binding.},
  author={Jianghong Li and David Rancour and M L Allende and Christopher A. Worth and Douglas S. Darling and Jacob Beal Seth Gilbert and Anant K. Menon and William W. Young},
  journal={Glycobiology},
  year={2001},
  volume={11 3},
  pages={217-29}
}
We tested the importance of the aspartate-any residue-aspartate (DXD) motif for the enzymatic activity and nucleotide binding capacity of the Golgi glycosyltransferase GM2 synthase. We prepared point mutations of the motif, which is found in the sequence 352-VLWVDDDFV, and analyzed cells that stably expressed the mutated proteins. Whereas the folding of the mutated proteins was not seriously disrupted as judged by assembly into homodimers, Golgi localization, and secretion of a soluble form of… CONTINUE READING

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Activity of the yeast MNN1 alpha-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases.

Proceedings of the National Academy of Sciences of the United States of America • 1998
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D. S. Darling, B. A. Macher, W. W. Young, Jr.
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