The DNA replication licensing system.

  title={The DNA replication licensing system.},
  author={Pia Thoemmes and J. Julian Blow},
  journal={Cancer surveys},
The Xenopus cell free system has proved a good model system to study in vitro DNA replication and the mechanism preventing rereplication in a single cell cycle. Studies using this system resulted in the development of a model postulating the existence of a replication licensing factor (RLF), which binds to the chromatin before the G1-S transition of the cell cycle and is displaced during replication. The nuclear envelope prevents rebinding of RLF and hence relicensing. Nuclear envelope… CONTINUE READING