The DNA-binding subunit p140 of replication factor C is upregulated in cycling cells and associates with G1 phase cell cycle regulatory proteins

  title={The DNA-binding subunit p140 of replication factor C is upregulated in cycling cells and associates with G1 phase cell cycle regulatory proteins},
  author={H. van der Kuip and B. Carius and S. J. Haque and B. Williams and C. Huber and T. Fischer},
  journal={Journal of Molecular Medicine},
The DNA-binding subunit of replication factor C (RFCp140) plays an important role in both DNA replication and DNA repair. The mechanisms regulating activation of RFCp140 thereby controlling replication and cellular proliferation are largely unknown. We analyzed protein expression of RFCp140 during cell cycle progression and investigated the association of RFCp140 with cell cycle regulatory proteins in cell lines of various tissue origin and in primary hematopoietic cells. Western and Northern… Expand
The Second Subunit of the Replication Factor C complex (RFC40) and the Regulatory Subunit (RI?) of Protein Kinase A form a Protein Complex Promoting Cell Survival
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Human DNA repair genes
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Proteomic Identi fi cation of a Direct Role for Cyclin D 1 in DNA Damage Repair
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Oxygen regulation of arterial smooth muscle cell proliferation and survival.
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  • Biology, Medicine
  • American journal of physiology. Heart and circulatory physiology
  • 2008
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Functions of replication factor C and proliferating-cell nuclear antigen: functional similarity of DNA polymerase accessory proteins from human cells and bacteriophage T4.
  • T. Tsurimoto, B. Stillman
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1990
A striking conservation of the DNA replication apparatus in human cells and bacteriophage T4 is demonstrated. Expand
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Deletion Analysis of the Large Subunit p140 in Human Replication Factor C Reveals Regions Required for Complex Formation and Replication Activities*
To characterize the role of the large subunit p140 in the function of the RFC complex, deletion mutants were created that defined a region within the p140 C terminus required for complex formation with the four small subunits. Expand
Structure-Function Relationship of the Eukaryotic DNA Replication Factor, Proliferating Cell Nuclear Antigen (*)
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The large subunit of replication factor C (Rfc1p/Cdc44p) is required for DNA replication and DNA repair in Saccharomyces cerevisiae.
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Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts.
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Saccharomyces cerevisiae replication factor C. II. Formation and activity of complexes with the proliferating cell nuclear antigen and with DNA polymerases delta and epsilon.
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  • Medicine, Biology
  • The Journal of biological chemistry
  • 1991
It is suggested that ATP binding promotes the formation of a labile complex of RF-C with PCNA at the primer terminus, whereas its hydrolysis is required to form a stable complex. Expand
Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4.
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Overexpression of mouse p140 subunit of replication factor C accelerates cellular proliferation.
The stable clones exhibited significantly accelerated cell proliferation, indicating that RFC-p140 is the limiting subunit of an active RFC complex in normal cells. Expand
Identification of Regions within the Four Small Subunits of Human Replication Factor C Required for Complex Formation and DNA Replication*
To study the individual subunits in the RFC complex and the role of the RFC boxes, deletion mutations were created in all subunits, suggesting unique functions for theindividual subunits. Expand