The DEXD/H-box RNA Helicase DDX19 Is Regulated by an α-Helical Switch*S⃞

@article{Collins2009TheDR,
  title={The DEXD/H-box RNA Helicase DDX19 Is Regulated by an $\alpha$-Helical Switch*S⃞},
  author={Ruairi Collins and Tobias Karlberg and Lari Lehti{\"o} and Patrick Schütz and Susanne van den Berg and L. G. Dahlgren and Martin Hammarström and John A. Weigelt and Herwig Sch{\"u}ler},
  journal={The Journal of Biological Chemistry},
  year={2009},
  volume={284},
  pages={10296 - 10300}
}
DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an α-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that… Expand
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