The Cytotoxic Action of Diphtheria Toxin and Its Degradation in Intact Vero Cells Are Inhibited by Bafilomycin Al , a Specific Inhibitor of Vacuolar-type H +-ATPase *

  • Toshiyuki Umata, Yoshinori MoriyamaS, +1 author Eisuke MekadaO
  • Published 2001
The role of vacuolar-type H+-ATPase (V-ATPase) in the cytotoxic action of diphtheria toxin (DT) was studied by using bafilomycin Al, a specific inhibitor of VATPase. Studies with acridine orange showed that the acidification of intracellular acidic compartments was inhibited strongly when Vero cells were treated with 600 nM bafilomycin Al, indicating that… CONTINUE READING