The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues

@inproceedings{Radomski2013TheCR,
  title={The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues},
  author={Nicolas Radomski and Louis Kreitmann and Fiona McIntosh and Marcel A. Behr},
  booktitle={PloS one},
  year={2013}
}
BACKGROUND Nucleic acid-based methods offer promise for both targeted and exploratory investigations of microbes in tissue samples. As the starting material for such studies is a mixture of host and microbial DNA, we have critically evaluated the DNA extraction step to determine the quantitative and qualitative parameters that permit faithful molecular detection of mycobacteria in infected tissue. Specifically, we assessed: 1) tissue disruption procedures; 2) DNA extraction protocols; and 3… CONTINUE READING
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