The Cohesin Complex Prevents the End Joining of Distant DNA Double-Strand Ends.

Abstract

The end joining of distant DNA double-strand ends (DSEs) can produce potentially deleterious rearrangements. We show that depletion of cohesion complex proteins specifically stimulates the end joining (both C-NHEJ and A-EJ) of distant, but not close, I-SceI-induced DSEs in S/G2 phases. At the genome level, whole-exome sequencing showed that ablation of RAD21 or Sororin produces large chromosomal rearrangements (translocation, duplication, deletion). Moreover, cytogenetic analysis showed that RAD21 silencing leads to the formation of chromosome fusions synergistically with replication stress, which generates distant single-ended DSEs. These data reveal a role for the cohesin complex in protecting against genome rearrangements arising from the ligation of distant DSEs in S/G2 phases (both long-range DSEs and those that are only a few kilobases apart), while keeping end joining fully active for close DSEs. Therefore, this role likely involves limitation of DSE motility specifically in S phase, rather than inhibition of the end-joining machinery itself.

DOI: 10.1016/j.molcel.2015.11.002

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Cite this paper

@article{Gelot2016TheCC, title={The Cohesin Complex Prevents the End Joining of Distant DNA Double-Strand Ends.}, author={Camille Gelot and Jos{\'e}e Guirouilh-Barbat and Tangui Le Guen and Elodie Dardillac and Catherine Chailleux and Yvan Canitrot and Bernard S Lopez}, journal={Molecular cell}, year={2016}, volume={61 1}, pages={15-26} }