• Biology
  • Published 2008

The Cloning and Analysis of Reverse Transcriptase of Ty1-copia-like Retrotransposons in Cucumis

@inproceedings{Wanping2008TheCA,
  title={The Cloning and Analysis of Reverse Transcriptase of Ty1-copia-like Retrotransposons in Cucumis},
  author={Zhang Wan-ping},
  year={2008}
}
Using degenerate oligoncleotide primers corresponding to conserved domains of the Ty1-copia retrantranspon reverse transcriptase,a fragment of 260 bp was amplified by PCR from Cucumis hystrix and C.sativus.The amplicons were cloned into pGEM-T Easy vector after purification,positive clones selected and identified by colony PCR,then sequenced and analyzed.Twenty-one different sequences of reverse transcriptase from Cucumis hystrix and C.sativus'Beijing Jietou' were obtained,and five families… CONTINUE READING