The ClC-3 chloride channel promotes acidification of lysosomes in CHO-K1 and Huh-7 cells.

  title={The ClC-3 chloride channel promotes acidification of lysosomes in CHO-K1 and Huh-7 cells.},
  author={Xinhua Li and Ting Wang and Zhifang Zhao and Steven A. Weinman},
  journal={American journal of physiology. Cell physiology},
  volume={282 6},
ClC-3 is a voltage-gated Cl- channel that is highly conserved and widely expressed, although its function, localization, and properties remain a matter of considerable debate. In this study, we have shown that heterologous expression of ClC-3 in either Chinese hamster ovary (CHO-K1) or human hepatoma (Huh-7) cells results in the formation of large, acidic vesicular structures within cells. Vesicle formation is prevented by bafilomycin, an inhibitor of the vacuolar ATPase, and is not induced by… CONTINUE READING
Highly Cited
This paper has 75 citations. REVIEW CITATIONS


Publications citing this paper.
Showing 1-10 of 47 extracted citations

Overexpression of CLC-3 in HEK293T cells yields novel currents that are pH dependent.

American journal of physiology. Cell physiology • 2008
View 5 Excerpts
Highly Influenced

Proton electrochemical gradient: Driving and regulating neurotransmitter uptake.

BioEssays : news and reviews in molecular, cellular and developmental biology • 2017

75 Citations

Citations per Year
Semantic Scholar estimates that this publication has 75 citations based on the available data.

See our FAQ for additional information.


Publications referenced by this paper.
Showing 1-10 of 40 references

Expression and canalicular localization of two isoforms of the ClC-3 chloride channel from rat hepatocytes.

American journal of physiology. Gastrointestinal and liver physiology • 2000
View 5 Excerpts
Highly Influenced

Disruption of ClC-3, a C1490 CLC-3 IN LYSOSOME ACIDIFICATION AJP-Cell Physiol

SM Stobrawa, T Breiderhoff, +9 authors TJ. Jentsch
Neuron 29: • 2002
View 3 Excerpts

ClC chloride channels in epithelia: recent progress and remaining puzzles.

News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society • 2001
View 1 Excerpt